PflM I (AccB7 I)

Origin:
Acinetobacter calcoaceticus B7

Concentration:
5000, u.a./ml

Recognition site:
CCANNNN/NTGG
GGTN/NNNNACC

Reaction buffer:
GM-buffer G, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 5-fold overdigestion with enzyme more than 95% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

BGOWY
10-2510025-5050-7550-75
Product
Cat. No.Size
E179S200 U
E179500 U
E1802500 U