Origin:
Acinetobacter calcoaceticus B7
Concentration:
5000, u.a./ml
Recognition site:
CCANNNN/NTGG
GGTN/NNNNACC
Reaction buffer:
GM-buffer G, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 5-fold overdigestion with enzyme more than 95% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
10-25 | 100 | 25-50 | 50-75 | 50-75 |
Product: | |
Cat. No. | Size |
E179S | 200 U |
E179 | 500 U |
E180 | 2500 U |