Origin:
Micrococcus species R9

Concentration:
10000 – 20000, u.a./ml

Recognition site:
CC/NGG
GGN/CC

Reaction buffer:
GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 2-fold overdigestion none of DNA fragments can be ligated.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 30 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 80°C

Enzyme activity in % of maximum:

B	G	Y	W	Y
50-75	50-75	100	50-75	75-100

Product :
Cat. No.	Qty.
E175S	200 U
E175	500 U
E176	2500 U