Sfc I (BstSF I, Sfe I)

Origin:
Bacillus stearothermophilus SF

Concentration:
2000 – 5000, u.a./ml

Recognition site:
C/TRYAG
GAYRT/C

Reaction buffer:
GM-buffer O + BSA, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100μg/ml BSA .)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 3-fold overdigestion with enzyme 95% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 5u.a. of enzyme for 16 hours at 60°C.

Optimum temperature:
60°C

Inactivation 20 minutes:
No

Enzyme activity in % of maximum:

BGOWY
75-10025-5010050-75 50-75
Product
Cat. No.Size
E197S100 U
E197200 U
E1981000 U