Origin:
Bacillus stearothermophilus SF
Concentration:
2000 – 5000, u.a./ml
Recognition site:
C/TRYAG
GAYRT/C
Reaction buffer:
GM-buffer O + BSA, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100μg/ml BSA .)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 3-fold overdigestion with enzyme 95% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 5u.a. of enzyme for 16 hours at 60°C.
Optimum temperature:
60°C
Inactivation 20 minutes:
No
Enzyme activity in % of maximum:
B | G | O | W | Y |
75-100 | 25-50 | 100 | 50-75 | 50-75 |
Product: | |
Cat. No. | Size |
E197S | 100 U |
E197 | 200 U |
E198 | 1000 U |