Origin:
E.coli strain that carries the cloned Pst I gene from Providencia stuartii

Concentration:
20000, u.a./ml

Recognition site:
CTGCA/G
G/ACGTC

Reaction buffer:
GM-buffer O + BSA, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA .)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 20-fold overdigestion with enzyme 90% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 80°C

Enzyme activity in % of maximum:

B	G	O	W	Y
10-25	25-50	100	25-50	25-50

Product :
Cat. No.	Size
E109S	2000 U
E109	5000 U
E110	25000 U