Origin:
E.coli strain that carries the cloned Pvu II gene from Proteus vulgaris
Concentration:
10000, u.a./ml
Recognition site:
CAG/CTG
GTC/GAC
Reaction buffer:
GM-buffer G + BSA, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT + 100μg/ml BSA .)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 10-fold overdigestion with enzyme more than 70% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 80°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
25-50 | 100 | 25-50 | 25-50 | 25-50 |
Product: | |
Cat. No. | Size |
E111S | 1000U |
E111 | 2000 U |
E112 | 10000 U |