Origin:
E.coli strain that caries the cloned Sph I gene from Streptomyces phaeochromogenes

Concentration:
5000, u.a./ml

Recognition site:
GCATG/C
C/GTACG

Reaction buffer:
GM-buffer G + BSA, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 50 mM NaCl; 1 mM DTT + 100 μg/ml BSA .)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 100 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 10-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
25-50	100	75-100	75-100	50-75

Product：
Cat. No.	Size
E129	200U
E130	1000 U