Sty I ( Erh I )

Origin:
Erwinia rhapontici

Concentration:
10000 – 50000, u.a./ml

Recognition site:
C/CWWGG
GGWWC/C

Reaction buffer:
GM-buffer 2W + BSA, (20 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 200 mM NaCl; 1 mM DTT + 100 μg/ml BSA.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 20-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 50 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65°C

Enzyme activity in % of maximum:

BGOWY
10-2525-5075-10075-100 10-25
Product
Cat. No.Size
E061S1000 U
E0615000 U
E06225000 U