:Origin Acinetobacter calcoaceticus :Concentration 1000 – 3000, u.a./ml :Recognition site AA/CGTT TTGC/AA :Reaction buffer GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ ml BSA.) :Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 […]
:Origin Bacillus stearothermophilus SC :Concentration 2000 – 5000, u.a./ml :Recognition site /CCNGG GGNCC/ :Reaction buffer GM-buffer Y, (33mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) :Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol. […]
:Origin Bacillus stearothermophilus P :Concentration 5000, u.a./ml :Recognition site G/CGCGC CGCGC/G :Reaction buffer GM-buffer G, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) :Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol. :Storage temperature -20°C :Ligation […]