:Origin
Acinetobacter calcoaceticus
:Concentration
1000 – 3000, u.a./ml
:Recognition site
AA/CGTT
TTGC/AA
:Reaction buffer
GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ ml BSA.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 0.05% Triton X-100, 50% glycerol.
Storage temperature: -20°C
:Ligation
After 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature: 37°C
Inactivation 20 minutes: under 65°C
:Enzyme activity in % of maximum
| B | G | O | W | Y |
| 0-10 | 0-10 | 0-10 | 0-10 | 100 |
| Product | |
| Cat. No. | Size |
| E011S | 100 U |
| E011 | 200 U |
| E012 | 1000 U |