Origin: E.coli strain that carries the cloned Tag I gene from Thermus aquaticus Concentration: 20000, u.a./ml Recognition site: T/CGA AGC/T Reaction buffer: GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); […]
Origin: Pseudomonas species N4 Concentration: 10000 – 30000, u.a./ml Recognition site: GGN/NCC CCN/NGG Reaction buffer: GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25℃); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% […]
Origin: E.coli strain that carries the cloned Tsp509 I gene from Sporosarcina species Concentration: 5000, u.a./ml Recognition site: /AATT TTAA/ Reaction buffer: GM-buffer B + BSA, (10 mM Tris-HCl(pH 7.6 at 25°C); 10 mM MgCl2;1 mM DTT + 100 μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM […]