Origin:
E.coli strain that carries the cloned Tsp509 I gene from Sporosarcina species
Concentration:
5000, u.a./ml
Recognition site:
/AATT
TTAA/
Reaction buffer:
GM-buffer B + BSA, (10 mM Tris-HCl(pH 7.6 at 25°C); 10 mM MgCl2;1 mM DTT + 100 μg/ml BSA.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 5-fold overdigestion with enzyme more than 95% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 55℃.
Optimum temperature:
55°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
100 | 75-100 | 50-75 | 50-75 | 75-100 |
Product: | |
Cat. No. | Size |
E217S | 200 U |
E217 | 500 U |
E218 | 2500 U |