Origin: Bacillus stearothermophillus MB Concentration: 5000 – 10000, u.a./ml Recognition site: /GATC CTAG/ Reaction buffer: GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 µg/ml BSA. Storage temperature: -20°C Ligation: After 5-fold […]
Origin: Pseudomonas pseudoalcaligenes Concentration: 500 – 1000, u.a./ml Recognition site: GAGTC(N)4/ CTCAG(N)5/ Reaction buffer: GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM […]
Origin: Micrococcus species R9 Concentration: 10000 – 20000, u.a./ml Recognition site: CC/NGG GGN/CC Reaction buffer: GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol. Storage […]