Origin:
Bacillus stearothermophillus MB
Concentration:
5000 – 10000, u.a./ml
Recognition site:
/GATC
CTAG/
Reaction buffer:
GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 µg/ml BSA.
Storage temperature:
-20°C
Ligation:
After 5-fold overdigestion with enzyme more than 95% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 65°C.
Optimum temperature:
65°C
Inactivation 20 minutes:
under 80°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
10-25 | 25-50 | 100 | 75-100 | 10-25 |
Product: | |
Cat. No. | Size |
E119 | 200 U |
E120 | 1000 U |