Origin: Acinetobacter calcoaceticus W2131 Concentration: 1000 – 3000, u.a./ml Recognition site: GGATC(N)4/ CCTAG(N)5/ Reaction buffer: GM-buffer Y + BSA, (33 mM Tris-acetate(pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM poatassium acetate; 1 mM DTT + 100 μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM […]
Origin: Pseudomonas species 124B Concentration: 10000 – 30000, u.a./ml Recognition site: GAGCT/C C/TCGAG Reaction buffer: GM-buffer G, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol. Storage […]
Origin: Curtobacterium citreus N Concentration: 2000 – 5000, u.a./ml Recognition site: GC/GGCCGC CGCCGG/CG Reaction buffer: GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% […]