Origin:
Acinetobacter calcoaceticus W2131
Concentration:
1000 – 3000, u.a./ml
Recognition site:
GGATC(N)4/
CCTAG(N)5/
Reaction buffer:
GM-buffer Y + BSA, (33 mM Tris-acetate(pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM poatassium acetate; 1 mM DTT + 100 μg/ml BSA.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol
Storage temperature: -20°C
Ligation:
After 2-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated with more than 500 u.a. of DNA ligase.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 6 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature: 37°C
Inactivation 20 minutes: under 65°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
75-100 | 50-75 | 0-10 | 0-10 | 100 |
Product | |
Cat. No. | Size |
E211 | 100 U |
E212 | 500 U |