Origin: E.coli strain that carries the cloned Sfa N I gene from Streptococcus faecalis N Concentration: 10000, u.a./ml Recognition site: GCATC(N)5/ CGTAG(N)9/ Reaction buffer: GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; […]
Origin: Pseudomonas species PP Concentration: 2000 – 5000, u.a./ml Recognition site: RG/GWCCY YCCWG/GR Reaction buffer: GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% […]
Origin: E.coli strain that carries the cloned PspOM I gene from Pseudomonas species OM2164 Concentration: 10000, u.a./ml Recognition site: G/GGCCC CCCGG/G Reaction buffer: GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Storage conditions: 20 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; […]