Origin:
Pseudomonas species PP
Concentration:
2000 – 5000, u.a./ml
Recognition site:
RG/GWCCY
YCCWG/GR
Reaction buffer:
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 5-fold overdigestion with enzyme more than 70% of DNA fragments can be ligated. Of these more than 80% can be recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
10-25 | 10-25 | 0-10 | 0-10 | 100 |
Product: | |
Cat. No. | Size |
E255 | 100 U |
E256 | 500 U |