Origin: Pseudomonas stutzeri N2 Concentration: 1000 – 3000, u.a./ml Recognition site: /(N)7GAACNNNNNNTAC(N)12/ /(N)12CTTGNNNNNNATG(N)7/ همهٔ نوشتهها Reaction buffer: GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA .) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 […]
Origin: Enterobacter gergoviae Concentration: 5000 – 10000, u.a./ml Recognition site: GGC/GCC CCG/CGG Reaction buffer: GM-buffer B + BSA, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT + 100 μg/ml BSA .) Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Storage […]
Origin: E.coli strain that carries the cloned Pst I gene from Providencia stuartii Concentration: 20000, u.a./ml Recognition site: CTGCA/G G/ACGTC Reaction buffer: GM-buffer O + BSA, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA .) Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 […]