Origin:
Pseudomonas stutzeri N2
Concentration:
1000 – 3000, u.a./ml
Recognition site:
/(N)7GAACNNNNNNTAC(N)12/
/(N)12CTTGNNNNNNATG(N)7/
Reaction buffer:
GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA .)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 2-fold overdigestion with enzyme
more than 70% of DNA fragments can be ligated. Of these 80% can be
recut. In the presense of 10% PEG ligation is better.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 30°C.
Optimum temperature:
30°C
Inactivation 20 minutes:
under 65°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
10-25 | 10-25 | 0-10 | 0-10 | 100 |
Product : | |
Cat. No. | Size |
E131 | 100U |
E132 | 500 U |