Origin: E.coli strain that carries the cloned Sma I gene from Serratia marcescens Concentration: 20000, u.a./ml Recognition site: CCC/GGG GGG/CCC Reaction buffer: GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; […]
Concentration: 5000 – 10000, u.a./ml Origin: Bacillus stearothermophilus SN Recognition site: TAC/GTA ATG/CAT Reaction buffer: GM-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol. Storage temperature: -20°C Ligation: […]
Origin: Pseudomonas lemoignei 19 Concentration: 2000 – 5000, u.a./ml Recognition site: CGAT/CG GC/TAGC Reaction buffer: GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% […]