Concentration:
5000 – 10000, u.a./ml
Origin:
Bacillus stearothermophilus SN
Recognition site:
TAC/GTA
ATG/CAT
Reaction buffer:
GM-buffer B, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 5-fold overdigestion with enzyme more than 70% of DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 80°C
Enzyme activity in % of maximum:
B | G | O | W | Y |
100 | 50-75 | 0-10 | 10-25 | 50-75 |
Product: | |
Cat. No. | Size |
E065 | 200 U |
E066 | 1000 U |