:Origin Deinococcus radiodurans EA :Concentration 5000 – 10000, u.a./ml :Recognition site GACNNN/NNGTC CTGNN/NNNCAG :Reaction buffer GM-buffer Y , (33 mM Tris-acetate(pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM poatassium acetate; :Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol Storage temperature: -20°C :Ligation After […]
:Origin Agrococcus species 25 Concentration 5000 u.a./ml :Recognition site TTS/AA AA/STT :Reaction buffer GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM Magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) :Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7mM 2-mercaptoethanol; 200 ug/ml BSA;50% glycerol. Storage […]
:Origin Arthrobacter species G :Concentration 3000 – 5000, u.a./ml :Recognition site A/CCGGT TGGCC/A :Reaction buffer GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 1 mM 2-mercaptoethanol; 50% glycerol +100μg/ ml BSA. Storage […]