:Origin
Agrococcus species 25

Concentration
5000  u.a./ml

:Recognition site
TTS/AA
AA/STT

:Reaction buffer
GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM Magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7mM 2-mercaptoethanol; 200 ug/ml BSA;50% glycerol.

Storage temperature: -20°C

:Ligation
After 5-fold overdigestion with enzyme more than >90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

:Enzyme activity in % of maximum

B	G	O	W	Y
75-100	50-75	10-25	10-25	100

Product
Cat. No.	Size
E573S	100 U
E573	200 U
E574	1000 U