:Origin
E. coli strain that carries Aat II gene from Acetobacter aceti
:Concentration
5000 – 10000, u.a./ml
:Recognition site
GACGT/C
C/TGCAG
:Reaction buffer
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol
Storage temperature: -20°C
:Ligation
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Optimum temperature: 37°C
Inactivation 20 minutes: under 65°C
Enzyme activity in GM buffer by %:
B | G | O | W | Y |
10-25 | 25-50 | 10-25 | 25-50 | 100 |
Product | |
Cat. No. | Size |
E287S | 100 U |
E287 | 500 U |
E288 | 2500 U |