:Origin
E. coli strain that carries Aat II gene from Acetobacter aceti

:Concentration
5000 – 10000, u.a./ml

:Recognition site
GACGT/C
C/TGCAG

:Reaction buffer
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol

Storage temperature: -20°C

:Ligation
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

Enzyme activity in GM buffer by %:

B	G	O	W	Y
10-25	25-50	10-25	25-50	100

Product
Cat. No.	Size
E287S	100 U
E287	500 U
E288	2500 U