:Origin
E.coli strain that carries Fbl I gene from Flavobacterium balustinum
:Concentration
1000 – 2000, u.a./ml
:Recognition site
GT/MKAC
CAKM/TG
:Reaction buffer
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol
Storage temperature: -20°C
:Ligation
After 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 3 u.a. of enzyme for 16 hours at 55°C.
Optimum temperature: 55°C
Inactivation 20 minutes: under 80°C
:Enzyme activity in GM buffer by %
B | G | O | W | Y |
50-75 | 75-100 | 0-10 | 50-75 | 100 |
Product | |
Cat. No. | Size |
E271S | 100 U |
E271 | 200 U |
E272 | 1000 U |