:Origin
E.coli strain that carries Fbl I gene from Flavobacterium balustinum

:Concentration
1000 – 2000, u.a./ml

:Recognition site
GT/MKAC
CAKM/TG

:Reaction buffer
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol

Storage temperature: -20°C

:Ligation
After 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 3 u.a. of enzyme for 16 hours at 55°C.

Optimum temperature: 55°C

Inactivation 20 minutes: under 80°C

:Enzyme activity in GM buffer by %

B	G	O	W	Y
50-75	75-100	0-10	50-75	100

Product
Cat. No.	Size
E271S	100 U
E271	200 U
E272	1000 U