:Origin
Acinetobacter calcoaceticus 65

:Concentration
10000 – 30000, u.a./ml

:Recognition site
C/GTACC
CCATG/G

:Reaction buffer
GM-buffer W, (10 mM Tris-acetate (pH 8.5 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol

Storage temperature: -20°C

:Ligation
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 30 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

:Enzyme activity in GM buffer by %

B	G	O	W	Y
50-75	75-100	0-10	50-75	100

Product
Cat. No.	Size
E003S	250 U
E003	1000 U
E004	5000 U