Origin:
Bacillus species 1720
Concentration:
10000, u.a./ml
Recognition site:
GC/TNAGC
CGANT/CG
Reaction buffer:
GM-buffer G , (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2 ; 50 mM NaCl; 1 mM DTT .)
Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol
Storage temperature:
-20°C
Ligation:
After 10-fold overdigestion with enzyme more than 80% of the DNA fragments can be ligated. Of these about 95% can be recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature:
37°C
Inactivation 20 minutes:
under 80 °C
Enzyme activity in % of maximum:
B | G | O | W | Y |
50-75 | 100 | 50-75 | 50-75 | 75-100 |
Product: | |
Cat. No. | Size |
E185S | 100 U |
E185 | 200 U |
E186 | 1000 U |