:Origin
E. coli strain that carries the cloned Acu I gene from Acinetobacter calcoaceticus SRW4
:Concentration
500 – 2000, u.a./ml
:Recognition site
CTGAAG(N)16/
GACTTC(N)14/
:Reaction buffer
GM-buffer Y + SAM + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 0.01 mM S-adenosylmethionine + 100 μg/ml BSA.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 µg/ml BSA; 50% glycerol
Storage temperature: -20°C
:Ligation
After 2-fold overdigestion with enzyme more than 80% of the DNA fragments can be ligated and recut.
:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature: 37°C
Inactivation 20 minutes: under 65°C
:Enzyme activity in % of maximum
| B | G | O | W | Y |
| 25-50 | 50-75 | 50-75 | 75-100 | 100 |
| Product | |
| Cat. No. | Size |
| E451 | 50 U |
| E452 | 250 U |