:Origin
Arthrobacter species G

:Concentration
3000 – 5000, u.a./ml

:Recognition site
A/CCGGT
TGGCC/A

:Reaction buffer
GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 1 mM 2-mercaptoethanol; 50% glycerol +100μg/ ml BSA.

Storage temperature: -20°C

:Ligation
After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

:Enzyme activity in % of maximum

B	G	O	W	Y
10-25	25-50	100	75-100	10-25

Product
Cat. No.	Size
E235	100 U
E236	500 U