:Origin
Deinococcus radiodurans EA
:Concentration
5000 – 10000, u.a./ml
:Recognition site
GACNNN/NNGTC
CTGNN/NNNCAG
:Reaction buffer
GM-buffer Y , (33 mM Tris-acetate(pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM poatassium acetate;
:Storage conditions
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol
Storage temperature: -20°C
:Ligation
After 5-fold overdigestion with enzyme more than 5% of the DNA fragments can be ligated. Ligation is better in presence of 10% PEG.
:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature: 37°C
Inactivation 20 minutes: under 65°C
:Enzyme activity in % of maximum
B | G | O | W | Y |
75-100 | 75-100 | 10-25 | 10-25 | 100 |
Product | |
Cat. No. | Size |
E193 | 200 U |
E194 | 1000 U |