Origin:
Acinetobacter calcoaceticus W2131

Concentration:
1000 – 3000, u.a./ml

Recognition site:
GGATC(N)4/
CCTAG(N)5/

Reaction buffer:
GM-buffer Y + BSA, (33 mM Tris-acetate(pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM poatassium acetate; 1 mM DTT + 100 μg/ml BSA.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol

Storage temperature: -20°C

Ligation:
After 2-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated with more than 500 u.a. of DNA ligase.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 6 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
75-100	50-75	0-10	0-10	100

Product
Cat. No.	Size
E211	100 U
E212	500 U