Origin:
Acetobacter pasteurianus

Concentration:
10000, u.a./ml

Recognition site:
GGGCC/C
C/CCGGG

Reaction buffer:
GM-buffer Y + BSA, (33mM Tris-acetate(pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM poatassium acetate; 1 mM DTT + 100 μg/ml BSA.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol

Storage temperature: -20°C

Ligation:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

Enzyme activity in % of maximum:

B	G	O	W	Y
50-75	25-50	0-10	0-10	100

Product
Cat. No.	Size
E019S	1000 U
E019	5000 U
E020	25000 U