:Origin
Pseudomonas alcaligenes BS17

:Concentration
500 – 2000, u.a./ml

:Recognition site
GG/CGCGCC
CCGCGC/GG

:Reaction buffer
GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol

Storage temperature: -20°C

:Ligation
After 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

:Enzyme activity in % of maximum

B	G	O	W	Y
25-50	10-25	0-10	0-10	100

Product
Cat. No.	Size
E483S	100 U
E483	500 U
E484	2500 U