:Origin
Bacillus megaterium 18
:Concentration
5000 – 20000, u.a./ml
:Recognition site
G/GWCC
CCWG/G
:Reaction buffer
GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol
Storage temperature: -20°C
:Ligation
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature: 37°C
Inactivation 20 minutes: under 65°C
:Enzyme activity in % of maximum
B | G | O | W | Y |
10-25 | 25-50 | 100 | 75-100 | 10-25 |
Product | |
Cat. No. | Size |
E029S | 400 U |
E029 | 1000 U |
E030 | 5000 U |