:Origin
Arthrobacter species LE3860

:Concentration
20000, u.a./ml

:Recognition site
GCG/C
C/GCG

:Reaction buffer
GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol

Storage temperature: -20°C

:Ligation
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 80°C

:Enzyme activity in % of maximum

B	G	O	W	Y
10-25	75-100	100	50-75	25-50

Product
Cat. No.	Size
E221S	200 U
E221	1000 U
E222	5000 U