:Origin
E.coli strain that carries BamH I gene from Bacillus amyloliquefaciens H
:Concentration
20000 – 50000, u.a./ml
:Recognition site
G/GATCC
CCTAG/G
:Reaction buffer
GM-buffer G + BSA, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT + 100 μg/ml BSA.)
:Storage conditions
10 mM Tris-HCL(pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 100 µg/ml BSA; 50% glycerol
Storage temperature: -20°C
:Ligation
After 50-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Optimum temperature: 37°C
Inactivation 20 minutes: under 65°C
:Enzyme activity in % of maximum
B | G | O | W | Y |
25-50 | 100 | 75-100 | 75-100 | 25-50 |
Product: | |
Cat. No. | Size |
E021S | 2000 U |
E021 | 4000 U |
E022 | 20000 U |