Abs I

:Origin
Arthrobacter species 7M06

:Concentration
500-1000, u.a./ml

:Recognition site
CC/TCGAGG
GGAGCT/CC

:Reaction buffer
GM-buffer AbsI (10 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 50 mM KCl; 1mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA;0,05% Triton X-100; 50% glycerol.

Storage temperature: -20°C

:Ligation
After 2-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.

Optimum temperature: 37°C

Inactivation 20 minutes: 65°C

Enzyme activity in GM buffer by%

BGOWYR
75-10010-250-1050-750-1050
Product
Cat. No.Size
E53550 U
E536250 U