:Origin
Arthrobacter species 7M06
:Concentration
500-1000, u.a./ml
:Recognition site
CC/TCGAGG
GGAGCT/CC
:Reaction buffer
GM-buffer AbsI (10 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 50 mM KCl; 1mM DTT.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA;0,05% Triton X-100; 50% glycerol.
Storage temperature: -20°C
:Ligation
After 2-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
Optimum temperature: 37°C
Inactivation 20 minutes: 65°C
Enzyme activity in GM buffer by%
B | G | O | W | Y | R |
75-100 | 10-25 | 0-10 | 50-75 | 0-10 | 50 |
Product | |
Cat. No. | Size |
E535 | 50 U |
E536 | 250 U |