:Origin
Arthrobacter species A2

:Concentration
10000 – 20000, u.a./ml

:Recognition site
C/CTAGG
GGATC/C

:Reaction buffer
GM-buffer W + BSA, (10mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA ; 50% glycerol

Storage temperature: -20°C

:Ligation
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

:Enzyme activity in % of maximum

B	G	O	W	Y
10-25	50-75	75-100	100	75-100

Product
Cat. No.	Size
E245S	100 U
E245	500 U
E246	2500 U