:Origin
E.coli strain that carries BamH I gene from Bacillus amyloliquefaciens H

:Concentration
20000 – 50000, u.a./ml

:Recognition site
G/GATCC
CCTAG/G

:Reaction buffer
GM-buffer G + BSA, (10mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl₂; 50 mM NaCl; 1 mM DTT + 100 μg/ml BSA.)

:Storage conditions
10 mM Tris-HCL(pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 100 µg/ml BSA; 50% glycerol

Storage temperature: -20°C

:Ligation
After 50-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 37°C.

Optimum temperature: 37°C

Inactivation 20 minutes: under 65°C

:Enzyme activity in % of maximum

B	G	O	W	Y
25-50	100	75-100	75-100	25-50

Product：
Cat. No.	Size
E021S	2000 U
E021	4000 U
E022	20000 U