Origin:
Bacillus pumilus

Concentration:
200, u.a./ml

Recognition site:
CTGGAG(N)₁₆/
GACCTC(N)₁₄/

Reaction buffer:
GM-buffer W + BSA, (10 mM Tris-HCl(pH 8.5 at 25°C); 10 mM MgCl₂ ;100 mM NaCl; 1 mM DTT + 100 μg/ml BSA).

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 2-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and 95% may be recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 0.4 u.a. of enzyme for 1 hour at 37°C.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65 °C

Enzyme activity in % of maximum:

B	G	O	W	Y
25-50	50-70	75-100	100	50-75

Product：
Cat. No.	Size
E467	50 U
E468	250 U