Origin:
Bacillus megaterium S87

Concentration:
500-1000, u.a./ml

Recognition site:
ACTGGG(N)₅/
TGACCC(N)₄/

Reaction buffer:
GM-buffer Y, (33 mM Tris acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT .)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 2-fold overdigestion with enzyme 75% of the DNA fragments can be ligated and 95% may be recut.

Non-specific hydrolysis:
Overnight digest with Bmr I is not recommended. A 50 μl reaction containing 1μg of DNA and 0.5 units of enzyme incubated for 4 hours resulted in the same pattern of DNA bands as a reaction incubated for 1 hour.

Optimum temperature:
37°C

Inactivation 20 minutes:
under 65 °C

Enzyme activity in % of maximum:

B	G	O	W	Y
75-100	75-100	25-50	10-25	100

Product：
Cat. No.	Size
E487	25 U
E488	125 U