Origin:
Bacillus schlegelii 4

Concentration:
10000 – 20000, u.a./ml

Recognition site:
CCNNNNN/NNGG
GGNN/NNNNNCC

Reaction buffer:
GM-buffer W + BSA, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA.)

Storage conditions:
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

Storage temperature:
-20°C

Ligation:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 30 u.a. of enzyme for 16 hours at 55°C.

Optimum temperature:
55°C

Inactivation 20 minutes:
under 80 °C

Enzyme activity in % of maximum:

B	G	O	W	Y
75-100	75-100	50-75	100	25-50

Product：
Cat. No.	Size
E219S	100 U
E219	500 U
E220	2500 U