Origin:
Bacillus stearothermophilus MA
Concentration:
30000-100000, u.a./ml
Recognition site:
GTCTC(N)1/
CAGAG(N)5/
Reaction buffer:
GM-buffer W + BSA, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA. )
Storage conditions:
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol.
Storage temperature:
-20°C
Ligation:
After 50-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolysis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 100 u.a. of enzyme for 16 hours at 55°C.
Optimum temperature:
55°C
Inactivation 20 minutes:
under 65 °C
Enzyme activity in % of maximum:
B | G | O | W | Y |
25-50 | 50-75 | 50-75 | 100 | 75-100 |
Product: | |
Cat. No. | Size |
E291S | 200 U |
E291 | 1000 U |
E292 | 5000 U |