:Origin
Bacillus stearothermophilus AU

:Concentration
20000, u.a./ml

:Recognition site
T/GTACA
ACATG/T

:Reaction buffer
GM-buffer W, (10mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl₂; 100 mM NaCl; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 80°C

:Enzyme activity in % of maximum

B	G	O	W	Y
10-25	50-75	25-50	100	25-50

Product：
Cat. No.	Size
E267S	100 U
E267	500 U
E268	2500 U