:Origin
Bacillus stearothermophilus AU
:Concentration
20000, u.a./ml
:Recognition site
T/GTACA
ACATG/T
:Reaction buffer
GM-buffer W, (10mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol.
:Storage temperature
-20°C
:Ligation
After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 37°C.
:Optimum temperature
37°C
:Inactivation 20 minutes
under 80°C
:Enzyme activity in % of maximum
B | G | O | W | Y |
10-25 | 50-75 | 25-50 | 100 | 25-50 |
Product: | |
Cat. No. | Size |
E267S | 100 U |
E267 | 500 U |
E268 | 2500 U |