:Origin
Bacillus stearothermophilus SC

:Concentration
2000 – 5000, u.a./ml

:Recognition site
/CCNGG
GGNCC/

:Reaction buffer
GM-buffer Y, (33mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 3 u.a. of enzyme for 16 hours at 55°C.

:Optimum temperature
55°C

:Inactivation 20 minutes
under 80°C

:Enzyme activity in % of maximum

B	G	O	W	Y
50-75	50-75	50-75	50-75	100

Product：
Cat. No.	Size
E307	100 U
E308	500 U