:Origin
Bacillus stearothermophilus 2B
:Concentration
5000 – 10000, u.a./ml
:Recognition site
C/TCGTG
GAGCA/C
:Reaction buffer
GM-buffer Y + BSA, (33mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 μg/ml BSA.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol.
:Storage temperature
-20°C
:Ligation
After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 60°C.
:Optimum temperature
60°C
:Inactivation 20 minutes
under 80°C
:Enzyme activity in % of maximum
| B | G | O | W | Y |
| 75-100 | 25-50 | 10-25 | 25-50 | 100 |
| Product： | |
| Cat. No. | Size |
| E043S | 100 U |
| E043 | 200 U |
| E044 | 1000 U |