Dpn I ( Mal I )

:Origin
Marinococus albus I

:Concentration
500 – 1000, u.a./ml

:Recognition site
G(mA)/TC
CT/(mA)G

:Reaction buffer
GM-buffer Dpn I, (20mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 150 mM NaCl; 1 mM DTT.)

:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol.

:Storage temperature
-20°C

:Ligation
After 2 -fold overdigestion with enzyme more than 80% of the DNA fragments can be ligated and recut.

:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.

:Optimum temperature
37°C

:Inactivation 20 minutes
under 65°C

Product
Cat. No.Size
E489S100 U
E489500 U
E4902500 U