:Origin
Marinococus albus I
:Concentration
500 – 1000, u.a./ml
:Recognition site
G(mA)/TC
CT/(mA)G
:Reaction buffer
GM-buffer Dpn I, (20mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 150 mM NaCl; 1 mM DTT.)
:Storage conditions
10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol.
:Storage temperature
-20°C
:Ligation
After 2 -fold overdigestion with enzyme more than 80% of the DNA fragments can be ligated and recut.
:Non-specific hydrolysis
No nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37°C.
:Optimum temperature
37°C
:Inactivation 20 minutes
under 65°C
Product: | |
Cat. No. | Size |
E489S | 100 U |
E489 | 500 U |
E490 | 2500 U |